cumulative frequency histograms (Oxford Instruments)
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cumulative frequency histograms
![A quantitative analysis of postsynaptic DLG1 puncta for PNs and LNs in the DA1 glomerulus using dlg1[4K] (A) Schematic of the Drosophila antennal lobes showing presynaptic ORNs (orange) and postsynaptic PNs (magenta) in the DA1 glomerulus. (B) Representative confocal maximum projections of DA1 ORNs expressing Brp-Short-mStraw and DA1 PNs expressing DLG1-V5 and stained with antibodies against mStraw (red), V5 (green), and N-Cadherin (blue). (C) Single, high-magnification optical sections of the DA1 ORNs and PNs from (B). (D) Quantification of Brp-Short-mStraw puncta for ORNs and DLG1-V5 puncta for PNs. (E) <t>Cumulative</t> frequency histogram of the nearest-neighbor distance between DLG1-V5 puncta in DA1 PNs. The average NND (μ) and Cluster % of puncta are indicated. Gray traces represent individual glomeruli, and the green trace represents the aggregate average. (F) Schematic of the Drosophila antennal lobes showing presynaptic ORNs (orange) and postsynaptic LNs (blue) in the DA1 glomerulus. (G and H) Representative confocal image stacks and corresponding single, high-magnification sections of DA1 ORNs expressing Brp-Short-mStraw and multiglomerular LNs in the DA1 glomerulus expressing DLG1-V5 and stained with antibodies as in (B) and (C). (I) Quantification of Brp-Short-mStraw puncta for ORNs and DLG1-V5 puncta for LNs. (J) Cumulative frequency histogram of the nearest-neighbor distance between DLG1-V5 puncta in multiglomerular LNs in DA1, including the average (μ) and the cluster %. Traces represent individual glomeruli (gray) or the aggregate average (green). (K) Schematic of the Drosophila antennal lobes showing presynaptic PNs (magenta) and postsynaptic LNs (blue) in the DA1 glomerulus. (L and M) Representative confocal image stacks and corresponding single optical sections of DA1 PNs expressing Brp-Short-mStraw and multiglomerular LNs in the DA1 glomerulus expressing DLG1-V5 and stained with antibodies as in (B) and (C). (N) Quantification of Brp-Short-mStraw puncta for PNs and DLG1-V5 puncta for LNs. (O) Cumulative frequency histogram of the nearest-neighbor distance between DLG1-V5 puncta in the multiglomerular LNs from (L) and (M), including the average (μ) and the cluster %. Traces represent individual glomeruli (gray) or the aggregate average (green), as previously described. For all conditions, n ≥ 6 glomeruli from three brains, and 900 (E), 675 (J), or 450 (O) individual puncta. Scale bars, 5 μm.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1928/pmc10261928/pmc10261928__gr5.jpg)
Cumulative Frequency Histograms, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cumulative+frequency+histograms/Imaris/pmc10261928-484-6-12
Average 99 stars, based on 44395 article reviews
Cumulative Frequency Histograms, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 44395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cumulative+frequency+histograms/Imaris/pmc10261928-484-6-12
Average 99 stars, based on 44395 article reviews
cumulative frequency histograms - by Bioz Stars,
2026-10
99/100 stars
Images
1) Product Images from "A conditional strategy for cell-type-specific labeling of endogenous excitatory synapses in Drosophila"
Article Title: A conditional strategy for cell-type-specific labeling of endogenous excitatory synapses in Drosophila
Journal: Cell Reports Methods
doi: 10.1016/j.crmeth.2023.100477
Figure Legend Snippet: A quantitative analysis of postsynaptic DLG1 puncta for PNs and LNs in the DA1 glomerulus using dlg1[4K] (A) Schematic of the Drosophila antennal lobes showing presynaptic ORNs (orange) and postsynaptic PNs (magenta) in the DA1 glomerulus. (B) Representative confocal maximum projections of DA1 ORNs expressing Brp-Short-mStraw and DA1 PNs expressing DLG1-V5 and stained with antibodies against mStraw (red), V5 (green), and N-Cadherin (blue). (C) Single, high-magnification optical sections of the DA1 ORNs and PNs from (B). (D) Quantification of Brp-Short-mStraw puncta for ORNs and DLG1-V5 puncta for PNs. (E) Cumulative frequency histogram of the nearest-neighbor distance between DLG1-V5 puncta in DA1 PNs. The average NND (μ) and Cluster % of puncta are indicated. Gray traces represent individual glomeruli, and the green trace represents the aggregate average. (F) Schematic of the Drosophila antennal lobes showing presynaptic ORNs (orange) and postsynaptic LNs (blue) in the DA1 glomerulus. (G and H) Representative confocal image stacks and corresponding single, high-magnification sections of DA1 ORNs expressing Brp-Short-mStraw and multiglomerular LNs in the DA1 glomerulus expressing DLG1-V5 and stained with antibodies as in (B) and (C). (I) Quantification of Brp-Short-mStraw puncta for ORNs and DLG1-V5 puncta for LNs. (J) Cumulative frequency histogram of the nearest-neighbor distance between DLG1-V5 puncta in multiglomerular LNs in DA1, including the average (μ) and the cluster %. Traces represent individual glomeruli (gray) or the aggregate average (green). (K) Schematic of the Drosophila antennal lobes showing presynaptic PNs (magenta) and postsynaptic LNs (blue) in the DA1 glomerulus. (L and M) Representative confocal image stacks and corresponding single optical sections of DA1 PNs expressing Brp-Short-mStraw and multiglomerular LNs in the DA1 glomerulus expressing DLG1-V5 and stained with antibodies as in (B) and (C). (N) Quantification of Brp-Short-mStraw puncta for PNs and DLG1-V5 puncta for LNs. (O) Cumulative frequency histogram of the nearest-neighbor distance between DLG1-V5 puncta in the multiglomerular LNs from (L) and (M), including the average (μ) and the cluster %. Traces represent individual glomeruli (gray) or the aggregate average (green), as previously described. For all conditions, n ≥ 6 glomeruli from three brains, and 900 (E), 675 (J), or 450 (O) individual puncta. Scale bars, 5 μm.
Techniques Used: Expressing, Staining
Related Articles
Expressing:Article Title: Reflection-enhanced laser-induced fluorescence spectroscopy to improve the analytical sensitivity in liquids. Article Snippet: In brief Studying neuronal circuits requires concurrent preand postsynaptic labeling in identified subsets of neurons.. Parisi et al. create dlg1[4K], a CRISPRmodified dlg1 locus that conditionally labels DLG1 at excitatory postsynapses in vivo using cell-type-specific binary expression.. The dlg1[4K] label enables previously unavailable postsynaptic labeling and enhances quantitative circuit analysis. Article Title: A conditional strategy for cell-type-specific labeling of endogenous excitatory synapses in Drosophila Article Snippet: The individual values for “DistMin” and Staining:Article Title: Reflection-enhanced laser-induced fluorescence spectroscopy to improve the analytical sensitivity in liquids. Article Snippet: In brief Studying neuronal circuits requires concurrent preand postsynaptic labeling in identified subsets of neurons.. Parisi et al. create dlg1[4K], a CRISPRmodified dlg1 locus that conditionally labels DLG1 at excitatory postsynapses in vivo using cell-type-specific binary expression.. The dlg1[4K] label enables previously unavailable postsynaptic labeling and enhances quantitative circuit analysis. Article Title: A conditional strategy for cell-type-specific labeling of endogenous excitatory synapses in Drosophila Article Snippet: The individual values for “DistMin” and |